Fig. 4

RSV preserves mitochondria in the tubular shape in PA-treated HUVECs through TyrRS-PARP1 pathway. Cells were treated with 200 μM PA for 16 h, co-cultured with 10 μM RSV for another 8 h. a: Representative TEM images of HUVECs after treatment as above. Arrows indicate the lipid droplets, scale bars: 500 nm. b, d: Cells after the treatment above were labeled with MitoTracker. Representative images(b) were acquired by a laser scanning confocal microscope; scale bars: 500 nm. The percentage of cells without elongated mitochondria in each group were calculated (d). c, e: Cells were pretreated with siRNA of the vehicle, TyrRS or PAPR1 for 24 h before cultured with 200 μM PA for 16 h and co-cultured with 10 μM RSV for another 8 h. Cells then were labeled with MitoTracker. Representative images (c) were acquired by the laser scanning confocal microscope; scale bars: 500 nm. The percentage of cells without elongated mitochondria in each group were calculated (e). Values are expressed as means ± SD (n = 3); ^*p < 0.05, ^**p < 0.01 vs. the vehicle-treated control group; ^#p < 0.05, ^##p < 0.01 vs. vehicle + PA-treated group; ^$p < 0.05, ^$$p < 0.01 vs. vehicle+PA + RSV-treated group