Fig. 6
From: Myricetin improves endurance capacity by inducing muscle fiber type conversion via miR-499
miR-499/Sox6 axis is involved in inducing fast-to-slow fiber type transition by myricetin. After transfected with inhibitor or mimic of miR-499, the mRNA expressions of miR-499 (a), Myh7b (b), Myh7 (c), Sox6 (d), Myh1 (e), and Myh4 (f) in L6 myotubes were determined. Protein levels (g) of Myh7 and Sox6 were measured by immunoblotting. The mRNA (h) and protein (i) levels of PGC-1α were monitored in the transfected L6 myotubes. Oxygen consumption rates (OCR) of myotubes (j) were determined by Seahorse XFp metabolic flux analysis. Basal respiration, ATP production and maximal respiration (k) were analyzed. Data are presented as mean ± SD. *P<0.05, **P<0.01, ***P<0.001