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Fig. 2 | Nutrition & Metabolism

Fig. 2

From: Resveratrol attenuates endothelial oxidative injury by inducing autophagy via the activation of transcription factor EB

Fig. 2

RSV inhibited PA-induced injury in HUVECs. a Cells were pretreated with different concentrations (0.1, 1 and 10 μM) of RSV for 2 h. Then, 200 μM of PA was added for an additional 24 h period. Subsequently, cell viability was measured using a CCK-8 detection kit, as described in the Materials and methods section. b-d Cells were pretreated with 10 μM of RSV for 2 h. Then, 200 μM of PA was added for an additional 24 h period. The intracellular ROS levels were estimated using the probe DCFH-DA by a microplate reader (b). Also, the intracellular ROS levels were estimated by confocal microscopy (c) and the bar charts show quantification of the ROS generation levels expressed as the fold change (d). e The levels of MDA were detected using an MDA detection kit. Values are presented as means ± SD (n = 5); *p < 0.05, **p < 0.01 compared between the marked groups

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