Fig. 4

RSV increases TFEB activity in HUVECs. HUVECs were treated with different concentrations (0.1, 1, 10 μM) of RSV for 2 h. a The levels of nuclear and cytoplasmic TFEB were analyzed by western blotting, Histone H3 and β-actin were the standards for protein loading, respectively. b The bar graph shows the protein level quantification of nuclear TFEB (n-TFEB), cytoplasmic TFEB (c-TFEB) and the ratio of the endogenous nuclear TFEB to cytoplasmic TFEB. c The mRNA level of TFEB was then analyzed by qRT-PCR. d The mRNA levels of TFEB target genes were measured using qRT-PCR. Values are presented as means ± SD (n = 3); *p < 0.05, **p < 0.01 compared with control groups