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Fig. 5 | Nutrition & Metabolism

Fig. 5

From: Resveratrol attenuates endothelial oxidative injury by inducing autophagy via the activation of transcription factor EB

Fig. 5

RSV stimulated autophagy through activating TFEB in PA-treated HUVECs. Cells were pretreated with RSV (10 μM) for 2 h, then treatment with PA (200 μM) for another 24 h. Furthermore, TFEB was knocked-down by siRNA transfection in HUVECs as described in the Materials and methods section. a The mRNA levels of TFEB was detected in HUVECs by using qRT-PCR. b-c The bar graph shows the protein level quantification of nuclear TFEB (n-TFEB), cytoplasmic TFEB (c-TFEB) and the ratio of the endogenous nuclear TFEB to cytoplasmic TFEB were analyzed by western blotting (b) and the ratio displayed by bar graph (c). d-e The protein levels of LC3 I, LC3 II, P62 and LAMP1 were analyzed by western blotting (d) and quantification displayed by bar graph (e). f The mRNA levels of TFEB target genes were measured using qRT-PCR. g The cells were incubated with lysotracker red (50 nM, 15 min, at 37 °C) and immediately visualized by confocal microscopy. h The average LTR fluorescence was expressed as the MFI using IPP 6.0 software. Values are presented as means ± SD (n = 3); *p < 0.05, **p < 0.01 compared between the marked groups

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