Fig. 6

RSV attenuated oxidative injury through stimulating autophagy in a TFEB-dependent manner in PA-treated HUVECs. Cells were pretreated with RSV (10 μM) for 2 h, then treatment with PA (200 μM) for another 24 h. Furthermore, TFEB was knocked-down by siRNA transfection in HUVECs as described in the Materials and methods section. a-c Intracellular ROS levels were estimated using a probe DCFH-DA by confocal microscopy (a) and bar charts show quantification of the ROS generation levels expressed as the fold change (b), and by microplate reader, fluorescence was read at 485 nm for excitation and 520 nm for emission (c). d The levels of MDA were detected using an MDA detection kit. e The cell viability was measured using a CCK-8 detection kit, Values are presented as means ± SD (n = 6); *p < 0.05, **p < 0.01 compared between the marked groups