Fig. 2

PLIN5 and PA synergically accelerate LD formation. a Ad-PLIN5 transfection induced PLIN5 overexpression in INS-1 cells at mRNA (upper) and protein (down) level. b INS-1 cells were transfected with Ad-GFP or Ad-PLIN5, and 0.5 mM palmitate (PA) was loaded for 12 h. Subsequently, cells were fixed, stained with Nile red (red) to detect LD, and observed by confocal microscopy with the magnification of 200×. Nucleus stained with DAPI (blue). Nile red area was evaluated semiquantitatively by Image-Pro Plus 5.0 software from random 20 high-power fields and standardized by cell numbers. c The levels of ATGL, FAS were examined by western blot analysis and SREBP-1c by Q-PCR. Values are means ± SE from three independent experiments, each conducted in triplicate. * P < 0.05, **P < 0.01 vs. Ad-GFP group. # P < 0.05, vs. Ad-GFP + PA group. †P < 0.05, vs. Ad-PLIN5 group