Fig. 4

HFS + CUR fed mice maintain normal islet integrity. After 16 weeks of diet, a pancreas sections from mice on Control, HFS, or HFS + CUR diets were stained for insulin (green), glucagon (red), and DAPI (blue). Representative confocal images are shown using 40x oil objective. Pancreas⁺⁺ software was used to quantitate b total islet size (scale bar, 20 μm), c insulin contents (β-cell pixels), d glucagon contents (α-cell pixels), and e ratio of α-cell/β-cell. Quantitative analysis was based on at least 150 images per group (n = 8 mice per group). (^****p ≤ 0.0001 compared to Control; ^####p ≤ 0.0001 compared to HFS + CUR)