Fig. 5

Activation of caspase-9 in CRC cells after treatment with FFAE of krill oil. The expression of caspase-9 and cleaved caspase-9 was measured by western blotting in DLD-1 (A) and HT-29 (A’) cells following treatment with FFAE of krill oil at 0.03 μL/100 μL (containing 0.13 μM EPA/0.065 μM DHA) and 0.12 μL/100 μL (containing 0.52 μM EPA/0.26 μM DHA) for 4 h and 8 h. Fluorescent intensity of subcellular distribution of cleaved caspase-9 in DLD-1 (B-C) and HT-29 (B′-C′) cells was determined using a monoclonal antibody for cleaved caspase-9 following 8 h of treatment with FFAE of krill oil at 0.12 μL/100 μL(containing 0.52 μM EPA/0.26 μM DHA). Scale bar = 50 μM. Magnification = 60X. The results were verified through at least three individual experiments. Data are expressed as mean ± SEM. ***p < 0.001 compared to Ethanol control