Fig. 1

Effects of RIFV on the differentiation of human white adipocytes. a FITC-labeled RIFV was added to the culture medium of human preadipocytes for 48 h, and RIFV could successfully enter the human preadipocytes. b Human preadipocytes were cultured with RIFV (NC, 10 μM, 50 μM and 100 μM) and differentiated for 8 days to mature adipocytes. Then, the cells were stained with Oil Red O and visualized under a light microscope (× 200). c The cellular triglyceride contents of human mature adipocytes on differentiation day 8 were measured using a commercial TG assay kit. d The mRNA expression levels of the indicated genes C/EBP-β, C/EBP-α and PPAR-γ in mature human adipocytes treated with RIFV on day 4. Each value was normalized to PPIA. e The protein levels of the indicated genes C/EBP-β, C/EBP-α and PPAR-γ of mature human adipocytes treated with RIFV at day 4 of differentiation. Each value was normalized to β-actin. The data are expressed as the means ± SD, n = 3. *P < 0.05, **P < 0.01.Abbreviations: NC, negative control; C/EBP-β, CCAAT/enhancer binding protein beta; C/EBP-α, CCAAT/enhancer binding protein alpha; PPAR-γ, peroxisome proliferator-activated receptor gamma