Fig. 3

Effects of RIFV on the differentiation of mouse primary white adipocytes. a The mouse primary white preadipocytes were isolated and cultured with RIFV (NC, 10 μM, 50 μM and 100 μM) and then differentiated into mature adipocytes. The cells were stained with Oil Red O and visualized under a light microscope (× 200). b The cellular triglyceride contents of mouse mature adipocytes were measured by a commercial TG assay. c The mRNA expression levels of the indicated genes C/EBP-β, C/EBP-α, PPAR-γ, ATGL and FABP4 in mature mouse adipocytes treated with RIFV at day 4 of differentiation. Each value was normalized to PPIA. The data are expressed as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01.Abbreviations: ATGL, adipose triglyceride lipase; FABP4, fatty acid binding protein 4