Fig. 4

The human white adipocytes differentiation was inhibited by RIFV at the early stage. a–c The human preadipocytes were cultured with 50 μM RIFV from Day0 to Day3 during adipogenesis. Then the mature adipocytes were stained with Oil Red O and visualized under a light microscope (× 200) on differentiation Day8 a. The cellular triglyceride contents of mature adipocytes were measured b. The mRNA expression levels of FABP4 were analyzed by q-RT-PCR c. d–f The human adipocytes were cultured with 50 μM RIFV from Day6 to Day8 during adipogenesis. Then the mature adipocytes were stained with Oil Red O and visualized under a light microscope (× 200) on differentiation Day8 d. The cellular triglyceride contents of mature adipocytes were measured by a commercial TG assay e. The mRNA expression levels of FABP4 were analyzed by q-RT-PCR f. The data are expressed as the means ± SD of three independent experiments. **P < 0.01.ns. no significance