Table 2 Primer sequences used in quantitative real-time PCR assay
Primers | Accession No. | Sequences (5′ → 3′) | Product size (bp) |
|---|---|---|---|
PPAR-γ | NM_214379.1 | F-AACATTTCACAAGAGGTGACCA | 213 |
R-GATCTCGTGGACGCCATACT | |||
SREBP-1c | NM_214157.1 | F-AGCGGACGGCTCACAATG | 121 |
R-CGCAAGACGGCGGATTTA | |||
ACACA | NM_001114269.1 | F-ACATCCCCACGCTAAACA | 186 |
R-AGCCCATCACTTCATCAAAG | |||
FASN | NM_001099930.1 | F-GCTTGTCCTGGGAAGAGTGTA | 115 |
R-AGGAACTCGGACATAGCGG | |||
CD36 | NM_001044622.1 | F-ACCCTGAGACCCACACAGTC | 119 |
R-TACAGCTGCCACAGCCAGAT | |||
FABP4 | NM_001002817.1 | F-TGAAAGGTGTCACGGCTAC | 102 |
R-TCGGGACAATACATCCAACAGAG | |||
CPT-1A | XM_021091195.1 | F-ATTACGACGGCAGGCTGTTGAAG | 133 |
R- AATAGGCTTGGCGACACTTGGC | |||
HSL | NM_214315.3 | F-GCCCGAGACGAGATTAGC | 143 |
R-ATGAAGGGATTCTTGACGATG | |||
ATGL | no. EU373817 | F-CGCCAGCATCATCGAGGTGTC | 94 |
R-GCAGCCACGGATGGTCTTCAC | |||
DGAT1 | NM_214051.1 | F-GCTTCAGCCTTCTTCCACGAGTAC | 163 |
R-CGATGATGAGCGACAGCCACAC | |||
METTL3 | XM_003128580.5 | F-CCAGCACAGCTTCAGCAGTTCC | 129 |
R-TGGAGATGGCAAGACGGATGGAG | |||
METTL14 | XM_003129231.6 | F-GAGGAAGAGGTGGAACTTCTGCTG | 96 |
R-CTCCTCCACGGCCTCCTCTG | |||
WTAP | NM_001244241.1 | F-GCGGGAATAAGGCCTCCAAC | 136 |
R-TGTGAGTGGCGTGTGAGAGA | |||
FTO | NM_001112692.1 | F-GCAGAGCCGCCTACAACCTAAC | 142 |
R-ACCGCTGACCTGTCCACCAG | |||
ALKBH5 | XM_021067995.1 | F-GCAAGGTGAAGAGCGGCATCC | 128 |
R-GTCCACCGTGTGCTCGTTGTAC | |||
YTHDF1 | XM_021078235.1 | F-GTCTACCTGCTCTTCAGCGTCAAC | 139 |
R-AGATCCACTTCACGTCGAACTTGC | |||
YTHDF2 | XM_005665152.3 | F-CCACCTCCACCACAGCCTACTC | 140 |
R-CCAGCCTGAGACTGTCCTACTCC | |||
YTHDF3 | XM_021089300.1 | F-ATTGAGCAAGGCATGACTGGACTG | 140 |
R-GGCGCTGCACTGCTAACTGG | |||
β-actin | 397,563 | F-TGCGGGACATCAAGGAGAAG | 176 |
R-AGTTGAAGGTGGTCTCGTGG |