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Fig. 2 | Nutrition & Metabolism

Fig. 2

From: Dietary alterations modulate the microRNA 29/30 and IGF-1/AKT signaling axis in breast Cancer liver metastasis

Fig. 2

Diet modification using CR or FA repressed the IGF-1 pathway and altered the expression of miR-29 and miR-30. a Western blot analysis of IGF-1 pathway proteins from total protein purified from normal liver tissue of C57BL/6 J mice on AL, CR or FA diet. b qRT-PCR for expression of IGF-1 and IGF-1R in AL, CR, and FA normal liver tissue, mean ± SD (*- p < 0.05, n = 4). c Real time qPCR validation of miR-29b, miR-29c, miR-30a and miR-30b expression in normal liver of mice on AL, CR or FA diets, mean ± SD (*- p < 0.05, n = 4). d Expression of miR-29b (i), miR-29c (ii), miR-30a (iii) and miR-30b (iv) in primary breast cancer tumor of BALB/c mice on AL or CR (30%) diet represented by mean ± SE (***-p < 0.001 and *-p < 0.05, n = 3). e Expression of miR-29b (i), miR-29c (ii), miR-30a (iii) and miR-30b (iv) in liver tissue with metastases of breast cancer in BALB/c mice on AL or CR diets, represented by mean ± SE (***-p < 0.001 and *-p < 0.05, n = 3)

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