Fig. 5

Indirubin treatment ameliorated hepatic steatosis in HFD-induced obese mice. a Representative H&E staining (upper) and Oil-red staining (lower) images of liver tissue of mice fed NCD or HFD after 8-week treatment with either vehicle or indirubin. Scale bar = 50 μm. b Representive photographs of liver tissue in HFD-induced obese mice after 8-week treatment with either vehicle or indirubin. c Mean tissue weights of liver were measured in mice fed NCD or HFD after 8-week treatment with either vehicle or indirubin. d-e Liver TG d and TC e content, serum ALT g and AST h were measured in mice fed NCD or HFD after 8-week treatment with either vehicle or indirubin. f RT- qPCR analysis of genes related to adipogenesis and inflammation in livers of mice fed NCD or HFD after 8-week treatment with either vehicle or indirubin. g-h Serum ALT g and AST h were measured in mice fed NCD or HFD after 8-week treatment with either vehicle or indirubin. Data in c-h are presented as mean ± SD (n = 6 in each group). ^##p < 0.01, ^###p < 0.001 compared with NCD + vehicle group; ^*p < 0.05, ^**p < 0.01, ^***p < 0.01 compared with HFD + vehicle group