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Fig. 7 | Nutrition & Metabolism

Fig. 7

From: Indirubin, a small molecular deriving from connectivity map (CMAP) screening, ameliorates obesity-induced metabolic dysfunction by enhancing brown adipose thermogenesis and white adipose browning

Fig. 7

Indirubin promotes browning of sWAT by increasing BAT-specific markers and mitochondrial biogenesis in HFD-induced obese mice. a-f RT-qPCR analysis of genes related to thermogenesis a, fatty acid oxidation b, lipolysis c, common adipogenic marks d, beige cell markers e and mitochondrial biogenesis f in sWAT of mice fed NCD or HFD after 8-week treatment with either vehicle or indirubin. g Measurement of mtDMA copy number in sWAT of mice fed NCD or HFD after 8-week treatment with either vehicle or indirubin. h Immunohistochemistry (IHC) staining with a UCP1-specific antibody in BAT of mice fed NCD or HFD after 8-week treatment with either vehicle or indirubin. Scale bar = 50 μm. i-j Western blot analysis of proteins levels of UCP1, PGC1α, VDAC1, and OXPHOS in BAT of mice fed NCD (H) or HFD i after 8-week treatment with either vehicle or indirubin. β-tubulin serves as a loading control. Data in A-F are presented as mean ± SD (n = 6 in each group). #p < 0.05 compared with NCD + vehicle group;*p < 0.05, **p < 0.01 compared with HFD + vehicle group

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Nutrition & Metabolism

ISSN: 1743-7075

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