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Fig. 8 | Nutrition & Metabolism

Fig. 8

From: Indirubin, a small molecular deriving from connectivity map (CMAP) screening, ameliorates obesity-induced metabolic dysfunction by enhancing brown adipose thermogenesis and white adipose browning

Fig. 8

Indirubin enhances BAT activity and induces browning of WAT relying on PKA and p38MAPK signaling pathways. a Western blot analysis performed with the indicated antibodies (Phospho-PKA substrate, UCP1, PGC1α, p-CREB) in differentiated C3H10T1/2 cells on day 6 of brown adipogenesis in present or absent of indirubin and (or) PKA inhibitor. β-tubulin serves as a loading control. b Western blot analysis performed with the indicated antibodies (UCP1, PGC1α) in differentiated C3H10T1/2 cells on day 6 of brown adipogenesis in present or absent of indirubin and (or) p38 MAPK inhibitor. β-tubulin serves as a loading control. c Semi-quantitative analysis of p-p38/p38 ratio from (B). d Proposed model for the role of indirubin in activating BAT and browning of WAT, thus having benefit effects on ameliorating obesity and its associated metabolic dysfunction

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Nutrition & Metabolism

ISSN: 1743-7075

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