Fig. 2

The comparison of body composition between NCD and HFD feeding in Adult and Juvenile groups. a–j: Adult group (18 weeks old) A: BW at 66 days of NCD and HFD feeding (n = 4, 4). b: Representative image of NCD and HFD fed rats. c-f: Representative X-ray c, d and CT images e, f of NCD c, e and HFD d, f fed rats. CT images of E and F are sectional images of the white dotted lines (level of vertebra L5) in C and D, respectively. Yellow areas indicate subcutaneous fat, and pink areas indicate visceral fat. The BW of NCD and HFD fed rats scanned by CT (corresponding to the rats in Fig. B) was 336.7 g and 372.5 g, respectively. g: The percentage of total fat to BW (n = 4, 4). f: Subcutaneous and visceral fat weight in the NCD and HFD fed rats (n = 4, 4). I: Muscle weight in the NCD and HFD fed rats (n = 4, 4). j: The percentage of muscle weight to BW in the NCD and HFD fed rats (n = 4, 4). k–t: Juvenile group (13 weeks old). K: BW at 66 days of NCD or HFD feeding (n = 4, 4). l: Representative image of rats fed NCD and HFD. M-P: Representative X-ray m, n and CT images o, p of NCD m, o and HFD n, p fed rats. CT images of O and P are sectional images of the white dotted lines (level of vertebra L5) in M and N, respectively. Yellow areas indicate subcutaneous fat, and pink areas indicate visceral fat. The BW of NCD and HFD fed rats scanned by CT (corresponding to the rats in Fig. L) was 292.3 g and 284.3 g, respectively. q The percentage of total fat to BW (n = 4, 4). r: Subcutaneous and visceral fat weight in the NCD and HFD fed rats (n = 4, 4). S: Muscle weight in the NCD and HFD fed rats. (n = 4, 4) T: The percentage of muscle weight to BW in the NCD and HFD fed rats (n = 4, 4). * P < 0.05, ** P < 0.01. unpaired t-test