Fig. 1

Wild-type mice were fed with HFD or normal diet for 12 weeks, and liver change (a) was observed by H&E and oil red O staining. The TET family was detected by Q-PCR after total mRNA was extracted from the liver (b). After stimulating the cell steatosis with FFA-containing medium, the intracellular TG content was measured (d,f). The mRNA of the TET family in the cells was detected by a Q-PCR method, and normalized to β-actin (e,g). The content of TET1 in mouse liver and cells was detected by WB method (c,h,i). (* p < 0.05, *** p < 0.001). Intracellular lipid droplets BODIPY 493/503 (green) DNA stained with DAPI (blue), TET1 (red) (j)