Fig. 4

Disorder of ADMA signal pathway in the myocardium of T2DM rats. Levels of asymmetric dimethylarginine (ADMA) in serum and myocardium were measured in control and type 2 diabetic (T2DM) rats. Activities of dimethylarginine dimethylaminohydrolase (DDAH) and nitric oxide synthase (NOS) as well as content of nitric oxide (NO) reflected by its stable end products nitrite/nitrate were also detected; protein expressions of protein arginine N-methyltransferase 1 (PRMT1), DDAH 1 and DDAH2 as well as endothelial NOS (eNOS) were determined by Western blotting in the myocardium of control and T2DM rats. Panel A & B show the serum ADMA concentration and cardiac ADMA content; panel C displays the activities of DDAH and NOS, panel D presents nitrite/nitrate contents; panel E demonstrates the gel electrophoresis bands of indicated protein expression in the myocardium of rats, and panel F is the quantification of relative protein expression vs β-actin. Data are expressed as mean ± S.M.E., n = 5 ~ 6. ^*P < 0.05, ^**P < 0.01 vs Control