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Table 1 Macronutrient composition of the normal, high protein and high-fat diets which were fed to rats. Raw protein consists of casein and whey, raw fat comprises combinations of extra-virgin olive oil and butter and crude fiber consists of yellow shelled corn

From: High-protein and low-calorie diets improved the anti-aging Klotho protein in the rats’ brain: the toxic role of high-fat diet

Nutritional composition (g/100 g DM)

Normal

High-protein

High-fat

Raw protein

8.2

55.4

8.2

Raw fats

5.3

5.3

35.2

Crude fiber

47

4

47

Calcium

1

1

1

NaCl

5

5

5

Humidity

10

10

10

Methionine–cysteine

1.5

1

1.5

Sucrose

10

0

10

Lysine

5

11

5

vitamin mix AIN-93M

1

1

1

mineral mix AIN-93M

3.5

3.5

3.5

Soybean oil

7

10

7

Tallow

0

0

15