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Table 1 Primers used in qRT-PCR

From: MicroRNA-302a is involved in folate deficiency-induced apoptosis through the AKT-FOXO1-BIM pathway in mouse embryonic stem cells

 

Primer

Product size (bp)

miR-302a

RT primer: 5′GTCGTATCCAGTGCGTGTCGTGGA

GTCGGCAATTGCACTGGATACGACTCACCAA3′

67

F: 5′GGGGTAAGTGCTTCCATGTT3′

R: 5′CAGTGCGTGTCGTGGAGT3′

U6

RT primer: 5′CGCTTCACGAATTTGCGTGTCAT3′

89

F: 5′GCTTCGGCAGCACATATACTAAAAT3′

R: 5′CGCTTCACGAATTTGCGTGTCAT3′

AKT

F: 5′GGCTGCTCAAGAAGGACCCTAC3′

72

R: 5′GGTGCTGCATGATCTCCTTGG3′

FOXO1

F: 5′GTACAGCGCATAGCACCA3′

121

R: 5′GCGACAGACAGAGTTCCC3′

Bim

F: 5′GTTCTGCCGCCTTTCTG 3′

189

R: 5′TTCCCCATCTGCTGCTAA 3′

miR-302a Promoter

F: 5′GTTACCCTAATCTGTGCCATCAA3′

284

R: 5′AAGACGCCGACTTTAGACCAC3′

β-actin

F: 5′TTCCAGCCTTCCTTCTTG3′

182

R: 5′GGAGCCAGAGCAGTAATC3′