Fig. 6

S-Equol modulates PKA/PP2A activities in high glucose cultured INS-1 cells. INS-1 cells were treated for 48 h with or without S-Equol (0.1, 1 and 10 μM) in the continuous presence of high glucose (26.2 mM), and cells cultured with 5 mM of glucose denote the control. (a) Western blotting analysis of nuclear Chrebp; (b) The PP2A and (c) PKA activities. H, High glucose; S-Eq, S-Equol; Lane “1 – 5” represents Control, H), 0.1 μM S-Eq + H, 1 μM S-Eq + H and 10 μM S-Eq + H group respectively; PP2A,protein phophatase 2A; PKA, protein kinase A. Three to five independent experiments were run in each experiments and results were presented as means ± SD. *P < 0.05 vs. the Control group, ^# P < 0.05 vs. the High glucose group