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Table 2 Assessment of the bias of 25(OH)D3 detected by LC-MS/MS using NIST certified SRM-972a

From: Determination of serum 25-hydroxyvitamin D status among population in southern China by a high accuracy LC-MS/MS method traced to reference measurement procedure

SRM-972a
25(OH)D3
Certified values by NIST LC-MS/MS
Target values (ng/mL) Uncertainty Detected values (ng/mL) SD (n = 3) Bias(%)
Level 1 28.80 1.10 28.43 0.61 −1.27
Level 2 18.10 0.40 18.63 0.34 2.94
Level 3 19.80 0.40 20.09 0.23 1.47
Level 4 28.40 0.90 29.77 0.26 1.25
  1. SRM standard reference material, NIST National Institute of Standards & Technology