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Table 2 Assessment of the bias of 25(OH)D3 detected by LC-MS/MS using NIST certified SRM-972a

From: Determination of serum 25-hydroxyvitamin D status among population in southern China by a high accuracy LC-MS/MS method traced to reference measurement procedure

SRM-972a

25(OH)D3

Certified values by NIST

LC-MS/MS

Target values (ng/mL)

Uncertainty

Detected values (ng/mL)

SD (n = 3)

Bias(%)

Level 1

28.80

1.10

28.43

0.61

−1.27

Level 2

18.10

0.40

18.63

0.34

2.94

Level 3

19.80

0.40

20.09

0.23

1.47

Level 4

28.40

0.90

29.77

0.26

1.25

  1. SRM standard reference material, NIST National Institute of Standards & Technology
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Nutrition & Metabolism

ISSN: 1743-7075

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