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Fig. 3 | Nutrition & Metabolism

Fig. 3

From: Tetramethylpyrazine reverses high-glucose induced hypoxic effects by negatively regulating HIF-1α induced BNIP3 expression to ameliorate H9c2 cardiomyoblast apoptosis

Fig. 3

Effect of Tetramethylpyrazine on elevated HIF-1α and the downstream BNIP3 in H9c2 cells under hypoxia and high glucose condition. a Western blotting analysis (n = 3) show increase in both HIF1α and BNIP3 levels under hypoxia in high-glucose conditions. b Flow cytometry analysis (n = 3) show TMP induced modulation on the effect of hypoxia in H9c2 cells under high glucose condition; cells without labeling represents living (Q3) cells; the cells labeled with Annexin V-FITC (Q2, Q4) or propidium iodide (Q1, Q2) were respectively cells in apoptosis and in necrosis. c Microphotographs (400X magnification) of TUNEL assay show the ameliorating effect of TMP on cell death induced by hypoxia under high-glucose condition (n = 3)

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Nutrition & Metabolism

ISSN: 1743-7075

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