Fig. 1

The effect of the LC-SFA, palmitate, on pancreatic β-cell viability. INS-1E (a) and EndoC-βH1 (b) cells were treated with vehicle [0 µM], 125 µM, 250 µM or 500 µM C16:0 for 24 h or 72 h, respectively. EndoC-βH1 cells were exposed to 5.5 mM or 20 mM glucose (G) with or without 500 µM C16:0 for 72 h (c), or incubated for 72 h in DMEM/Ham’s F12 complete culturing medium with increasing concentrations of C16:0 [0–500 µM] (d). Cell death was assessed using flow cytometry after staining with propidium iodide. Dots represent individual data points from a minimum of three independent experiments and the histograms represent mean values + SEM. *** p < 0.001 relative to vehicle [0 µM]