Fig.4

The effect of LC-FFA on β-cell viability when co-incubated with pro-inflammatory cytokines. EndoC-βH1 cells were exposed to vehicle [0 µM] or 250 µM C16:0, C16:1 or C18:1 with and without a cytokine cocktail (cyt; 20 ng TNF-α, IL1-β, IFN-γ, and IL-6) for 48 h. Cell death was assessed using flow cytometry after staining with propidium iodide. Dots represent individual data points from a minimum of three independent experiments and the histograms represent mean values + SEM. **p < 0.01; ***p < 0.001 relative to cytokine [Cyt] vehicle [0 µM LC-FFA] control