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Fig. 2 | Nutrition & Metabolism

Fig. 2

From: The long noncoding RNA MALAT1 modulates adipose loss in cancer-associated cachexia by suppressing adipogenesis through PPAR-γ

Fig. 2

MALAT1 is required for adipogenesis. a Expression of MALAT1 in C3H10 cells transfected with ASO targeting MALAT1 (#1 and #2) or negative control (NC) and collected for qPCR analysis at differentiation day 6. b Representative images of ASO-treated C3H10 cells at differentiation day 6 labeled with Oil Red O (ORO) (scale bar, 100 μm). c Quantitative analysis of lipid accumulation in C3H10 cells from b at differentiation day 6. d qPCR analyses of the expression of adipogenic markers in ASO-treated C3H10 cells at differentiation day 6. e Immunoblot analyses of the expression of adipogenic markers in ASO-treated C3H10 cells at differentiation day 6. The experiments were repeated 3 times. The data are expressed as the means ± SEM. n = 3. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001

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Nutrition & Metabolism

ISSN: 1743-7075

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