Table 1 The name and sequence of the primers, the sizes, and annealing temperatures for each pair
Gene | Size (bp) | Sequence (5′→3′) | Annealing temperature (°C) |
|---|---|---|---|
GAPDH | 113 | F:CATGAGAAGTATGACAACAGCCT R:AGTCCTTCCACGATACCAAAGT | 58 |
PPARÆ” | 80 | F:CAGAAATGCCTTGCAGTGGG R:AACAGCTTCTCCTTCTCGGC | 59 |
CEBPα | 94 | F:TATAGGCTGGGCTTCCCCTT R:AGCTTTCTGGTGTGACTCGG | 60 |
CEBPβ | 154 | F:TTTGTCCAAACCAACCGCAC R:GCATCAACTTCGAAACCGGC | 59 |
SREBP1c | 117 | F:TCTCAGTCCCCTGGTCTCTG R:ATAGGCAGCTTCTCCGCATC | 59 |
INSIG2 | 114 | F:AGTGGTCCAGTGTAATGCGG R:TGGATAGTGCAGCCAGTGTG | 60 |
LPL | 137 | F:GCTCAGGAGCATTACCCAGTGTC R:GCTCCAAGGCTGTATCCCAAGA | 63 |
FASN | 107 | F:ATTCTGCCATAAGCCCTGTC R:CTGTGTACTCCTTCCCTTCTTG | 57 |