Fig. 4
Effects of CR on AKT/AS160/GLUT4 signaling. The diabetic Sprague–Dawley rats model was induced by feeding HFD for 8 weeks, followed by a single STZ injection (30 mg/kg). The rats were either provided HFD ab libitum (HFD + AL + STZ group) or fed HFD with a 30% restriction regimen (HFD + CR + STZ group). Rats given free access to normal chow diet served as a blank control (ND + AL group). All interventions lasted for 20 weeks. Effects of CR on the expressions of AKT, p-AKT, AS160, and GLUT4 were determined by western blotting. a Expressions of AKT, p-AKT, AS160 and GLUT4 in skeletal muscle tissue after 10 and 20 weeks of intervention. b, c Densitometric measurements of band intensity were performed using ImageJ 1.52q. d Expressions of AKT, p-AKT, AS160 and GLUT4 in white adipose tissue after 10 and 20 weeks of intervention. e, f Densitometric measurements of band intensity were performed using ImageJ 1.52q. Data were from at least three independent experiments and presented as the mean ± standard deviation (SD). *p < 0.05 between the two groups; CR, calorie restriction; HFD, high-fat diet; STZ, streptozotocin; ND + AL, normal diet provided ad libitum (BCT group); HFD + AL + STZ, HFD provided ad libitum and STZ injection (MCT group); HFD + CR + STZ, HFD with 30% CR and STZ injection (CR group)