Fig. 1

Dose-dependent effect of stearic acid (SA) on cell growth and viability in 1.1B4 cells (A). Effect of 1 mM SA on the level of caspase-2 (C2), cleaved caspase-9 (C9), cleaved caspase-8 (C8), cleaved caspase-7 (C7), cleaved caspase-6 (C6), cleaved caspase-3 (C3), and cleaved PARP in 1.1B4 cells, (B). Effect of 1 mM SA on the level of BiP and CHOP in 1.1B4 cells (C). Cells incubated without SA represented control cells. When assessing cell growth and viability (A), the number of living cells was determined after 48 h of incubation. Each column represents the mean of four separate cultures ± SEM. * p < 0.05 when comparing the number of control cells and cells treated with SA. The dotted line represents the number of cells of the inoculum. After 24 h of the incubation (B, C), the levels of individual proteins were determined using western blot analysis and the relevant antibodies (see “Materials and Methods”). Monoclonal antibody against human actin was used to confirm equal protein loading. The data shown were obtained in one representative experiment from at least three independent experiments