Fig. 3

High-fat diet after weaning increases anxiety and risk taking behavior. a, b Time spent exploring and distance in the open arms of the elevated plus maze was significantly increased in HFD as compared with CHD mice. c, d Time spent and total distance traveled in the inner zone of the open field were no significantly difference in HFD mice and CHD mice. e, f Time in food arm and food arm entries were no significantly difference in HFD mice and CHD mice in y-maze test. g Marble-burying behavior was significantly increased in HFD versus HFD mice. h Novelty-suppressed feeding was no significantly difference. i–l Quantification of the levels of protein to NeuN, BDNF, TH and AVP in hypothalamus by western blotting. Data are means ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001