Fig. 5

Comparison of energy metabolism and neurodevelopment between HF-C and CH-C groups. a Cumulative percentage of vaginal opening (VO). b Body weight, wet carcass and dry carcass weights in HF-C and CH-C group. c The weight of subcutaneous fat, gonadal fat and visceral fat in HFD and CHD groups. d The weight of ovaries and uterus were no difference between two groups. e Diet had no significant effects on the levels of serum estradiol. f, g Quantification of the levels of protein to GnRH and GnRH receptor in hypothalamus by western blotting. h, i Quantification of the levels of protein to kisspeptin1 and kisspeptin1 receptor in hypothalamus by western blotting. Data are means ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001