Fig. 2

The degrees of pro- and anti-proliferation were different between L6 and C2C12 myoblasts undertaken 15% and 20% stretches. L6 and C2C12 myoblasts were seeded onto flexible-bottomed 6-well plates and divided into CON, 15% stretch, and 20% stretch groups. After 24 h incubation, they were subjected to 15% or 20% stretch at 0.5 Hz duration for 6 h. Then the cell proliferation was determined by CCK8 at 24 h after stretch finished, so the duration of the entire experiment was ~ 54 h. The alteration degrees of 15% stretch-induced pro-proliferation and 20% stretch-induced anti-proliferation were compared between C2C12 and L6 myoblasts using the following formula: = (OD_{15% or 20% stretch}—OD_CON)/OD_CON. The experiments were repeated at least three times (mean ± SD, n = 3), * and ^# indicated p < 0.05 vs corresponding C2C12 cells