Fig. 4

KLF15 improves PA-induced abnormal glucose metabolism in 3T3-L1 adipocytes. 3T3-L1 adipocytes were dealt with different concentrations of PA (0, 150, 750, and 1500 μM) for 48 h. The mRNA (A–C) and protein D expression levels of GPR120, GPR40, P-p38 MAPK, and KLF15 were detected; Glucose consumption E of 3T3-L1 adipocytes was tested. The expression of KLF15 was then overexpressed or silenced by transfection with overexpression plasmid (OE-KLF15) or interference fragment (si-KLF15). The mRNA F–H, K–M expression levels of KLF15, ADIPOLIN, GLUT4 and glucose consumption J, O were detected. After overexpression or silencing of KLF15 in mature adipocytes stimulated by PA for 48 h, the cells were continuously stimulated with 100 nmol/L insulin solution, and insulin sensitivity I, N were tested. Results represent mean ± SEM. One-way ANOVA, *P < 0.05, **P < 0.01 versus the control group, ^#P < 0.05, ^##P < 0.05, ^###P < 0.001 versus the PA-treated group, difference was statistically significant