Fig. 3
Expression of cytochrome c and cleaved caspase-9 following treatments. The expression of cytochrome c (A) and cleaved caspase-9 (B) in tumours following different treatments determined immunohistochemically using monoclonal antibodies. Quantitative analysis of the fluorescence intensity of cytochrome c (C) and cleaved caspase-9 (D) expression in tumour tissues following various treatments determined by immunohistochemistry. Scale bar = 50 µM. Magnification = 40X. N = 6 mice per group. The level of fluorescence was measured using 8 randomly taken images that provide a total area of 1 mm2. The results are expressed as mean ± SEM; *P < 0.05, ***P < 0.001 compared to the sham group; ^^^P  < 0.001 compared to the oxaliplatin-treated group. E The expression of cytochrome c and cleaved caspase-9 measured by western blotting in tumours from different treatment groups