Fig. 5
Expression of PD-L1 and PD-L2 following treatments. The expression of PD-L1 (A) and PD-L2 (B) in tumours following the treatments determined immunohistochemically using monoclonal antibodies. Quantitative analysis of the fluorescence intensity of PD-L1 (C) and PD-L2 (D) expression in tumour tissues following various treatments determined by immunohistochemistry. Scale bar = 50 µM. Magnification = 40X. N = 6 mice per group. The level of fluorescence was measured using 8 randomly taken images that provide a total area of 1 mm2. The results are expressed as mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001 compared to the sham group. E The expression of PD-L1 and PD-L2 measured by western blotting in tumours following various treatments