Fig. 4

PP2A inhibitor recovers high fructose-induced dysfunction mediated by NO reduction. MVECs were pretreated with or without 20 nM OA for 1 h and then stimulated with fructose. A NO production from MVECs (after pretreatment with fructose for 24 h) (n = 4). Western blot analysis showing the protein expression of eNOS, P-eNOS (Ser1177) (B, C), PP2AC, and P-PP2A (Tyr307) (D, E) in MVECs (after pretreatment with fructose for 8 h) (n = 3). Relative protein levels of P-eNOS (Ser1177) were normalized to that of eNOS, and those of P-PP2A (Tyr307) were normalized to that of PP2AC. All data are presented as the mean ± SEM. C, control; Fru, fructose. *P < 0.05, **P < 0.01 when compared with the respective control groups