Fig. 3

L-theanine inhibits fatty acid synthesis through down-regulating FASN, ACC1 and PPARγ. HepG2 and AML12 cells were treated with 500 μM OA for 24 h with or without pretreated L-theanine for 2 h. Western blot analysis showing protein expression of FASN, ACC1 and PPARγ in HepG2 (A) and AML12 (B) cells. C57BL/6J mice were fed normal control diet (NCD), HFD or HFD supplemented with L-Theanine for 16 weeks. C Real­time quantitative PCR analyses the levels of Fasn, Acaca and Pparγ mRNA expression in the mice liver. D Western blot analysis showing protein expression of FASN, ACC1 and PPARγ in mice liver. E Representative images of immunohistochemical staining of FASN, ACC1 and PPARγ in liver sections. Scale bar: 50 μm (40 ×). Band intensity was quantified by densitometry analysis. Values are expressed as mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 versus control or NCD group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 versus OA or HFD group