Fig. 5

DHM induces the browning program by upregulating IRF4/PGC-1α. A Protein levels of p-AMPK, T-AMPK, p-PKA, p-p38 MAPK, and T-p38 MAPK in iWAT. B Luciferase expression of the PGC-1α promoter in HEK293T cells. C Heatmaps representing the expression of thermogenic genes in iWAT by RNA-seq. D The IRF4 mRNA expression level in iWAT. (E) The IRF4 protein expression level in iWAT. F Luciferase expression of the IRF4 promoter in HEK293T cells. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001