Fig. 4

Effects of KCNQ1OT1 knockdown on the protective functions of Sal B in PA-induced ATDC5 cells. A Relative expression of KCNQ1OT1 in cartilage tissues obtained from normal group and OA group was determined by RT-qPCR. B Expression of KCNQ1OT1, C Cell viability, D–F Concentrations of pro-inflammatory cytokines in the culture supernatant, G and H Apoptotic ratio, and I and J expression levels of autophagy-related proteins were determined, after ATDC5 cells were in turn transfected with KCNQ1OT1 shRNA or Scr-shRNA, treated with 100 μM Sal B for 24 h, and treated with 500 μM PA for another 24 h. n = 3. Data are expressed as means ± SEM. Student’s t test and one-way ANOVA were used for the comparison in this study. *P < 0.05