Fig. 2

GlcN delays senescence of senile osteoporotic mice. A Comparison of adipocytes volume in the femoral bone marrow cavity of each group by HE staining (× 40). B Comparison of senescence serum maker expression (IL-1β, IL-6, MDA and SOD) for each group by serum biochemical test. C Western blot analysis was used to detect the expression of proliferation marker (Ki-67) and senescence maker (p16). D Immunohistochemistry analysis was used to detect the expression of Ki-67 in the distal femur (× 200). E Relative mRNAs expression of apoptotic markers (Bax, Bcl-2 and Cleaved Caspase-3) in bone tissue by qRT-PCR. F Relative proteins expression of Bax, Bcl-2 and Cleaved Caspase-3 in bone tissue by western blot