Fig. 6
From: Glucosamine delays the progression of osteoporosis in senile mice by promoting osteoblast autophagy
GlcN delays osteoporosis by promoting osteoblast autophagy. A Representative COL1A1 immunofluorescence staining (COL1A1 stained with green, DAPI stained with blue) in proximal tibia (× 100). B Relative mRNAs expression of osteogenesis-related genes (OCN, RUNX2 and BMP-2) in the bone tissue by qRT-PCR. C Relative proteins expression of OCN, RUNX2 and BMP-2 in the bone tissue by western blot. D Alkaline phosphatase staining in proximal tibia to exhibit the number of osteoblasts (the cytoplasm of osteoblasts was black granular) (× 100). E Goldner trichrome stain in proximal tibia to distinguish osteoid (stained with red) and mineralized bone (stained with green) (× 100)