Fig. 6
From: Analysis of different adipose depot gene expression in cachectic patients with gastric cancer
IRX1 suppresses adipogenesis in adipocytes. A Relative IRX1 expression with or without IRX1 overexpression. B, C qPCR and Western blot analysis of the expression of markers in adipocytes (6d) without/with overexpression of IRX1. D Oil red O staining of lipid accumulation in adipocytes (6d) without/with overexpression of IRX1 (Scale bar = 100 μm). E Relative IRX1 expression with or without IRX1 knockdown. F, G qPCR and Western blot analysis of the expression of markers in adipocytes (6d) without/with knockdown of IRX1. H Oil Red O staining of lipid accumulation in adipocytes (6d) without/with knockdown of IRX1 (Scale bar = 100 μm). * p < 0.05; ns indicates no significance