Table 1 Primer list
Genes | Forward primer | Reverse primer |
|---|---|---|
Emr1 | CTTTGGCTATGGGCTTCCAGTC | GCAAGGAGGACAGAGTTTATCGTG |
Fbxo32 | CTTTCAACAGACTGGACTTCTCGA | CAGCTCCAACAGCCTTACTACGT |
Fndc5 | ATGAAGGAGATGGGGAGGAA | GCGGCAGAAGAGAGCTATAACA |
Igatx | ATGTTGGTGGAAGCAAATGG | CCTGGGAATCCTATTGCAGA |
Il6 | ATGGATGCTACCAAACTGGAT | TGAAGGACTCTGGCTTTGTCT |
Mstn | CTGTAACCTTCCCAGGACCA | TCTTTTGGGTGCGATAATCC |
Myf5 | TGAGGGAACAGGTGGAGAAC | AGCTGGACACGGAGCTTTTA |
Myhc1 | CTCAGGTGGCTCCGAGAAAG | TGGCTGAGCCTTGGATTCTC |
Myhc2a | GCAAGAAGCAGATCCAGAAAC | GGTCTTCTTCTGTCTGGTAAGTAAGC |
Myhc2x | GCAACAGGAGATTTCTGACCTCAC | CCAGAGATGCCTCTGCTTC |
Myhc2b | TCTGGTAACACAAGAGGTGC | AAAAGGCTTGTTCTGGGCCT |
Myod1 | GCTGCCTTCTACGCACCTG | GCCGCTGTAATCCATCATGC |
Myog | CCAACCCAGGAGATCATTTG | ACGATGGACGTAAGGGAGTG |
Pgc1a | GCCCGGTACAGTGAGTGTTC | CTGGGCCGTTTAGTCTTCCT |
Pparg | TCGCTGATGCACTGCCTATG | TGTCAAAGGAATGCGAGTGGTC |
Tnfa | AGCCTGTAGCCCACGTCGTA | GGCACCACTAGTTGGTTGTCTTTG |
Trim63 | GGACTACTTTACTCTGGACTTAGAAC | CAGCCTCCTCTTCTGTAAACTC |
Ucp1 | TACCAAGCTGTGCGATGT | AAGCCCAATGATGTTCAGT |
Ucp3 | ATCGCCAGGGAGGAAGGA | GTTGACAATGGCATTTCTTGTGA |
Vdr | CACGGGCTTCCACTTCA | CGAGCAGGATGGCGATAA |
18Â s rRNA | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG |