Fig. 2

Hepatic mRNA and protein expression of Pcsk9 and Anxa2 genes after 20 weeks on HFD. The hepatic expression was assessed at mRNA and protein levels by qPCR/ELISA for PCSK9 (A, B) and qPCR/Western blot for ANXA2 (C, D). The mRNA level was calculated with 2^−ΔΔCt method. The relative protein level on the blots was determined by ImageJ, using actin as an internal control. Values are reported as mean ± SD; n = 5 in each group. The p value was determined by unpaired T-test. *p < 0.05, **p < 0.01, ***p < 0.001