Fig. 1

SPX ameliorates high-fat-diet-induced obesity in mice. (A) Schematic description of animal experiments. 3-week-old C57BL/6J male mice after one-week adaptive feeding were divided randomly into a high-fat diet group (HFD) and a normal diet (ND) group. After 12 weeks, HFD or ND mice were half received SPX injection (30 µg/kg/day; HFD + SPX or ND + SPX) or vehicle injection (HFD + Veh or ND + Veh). (B) The body weight during the protocol. (C) Obesity model successfully established after 12 weeks. (D, E) Body weight of mice after SPX or Veh treatment at the end of the study. (F) H&E staining in WAT (epididymal WAT) (scale bar: 100 μm). (G) Adipocyte size in WAT of the mice. (H) The food intake after SPX or Veh treatment among each group. All values are represented as means with error bars representing S.D. *, P < 0.05; **, P < 0.01. n = 6 for each group