Fig. 8

Exploration of therapeutic drugs and targets related to vitamin D metabolic activity. A: The predicted IC50 values of tumor cells against drugs for patients in the high- and low-risk groups, which compared by group T-test. B & C: The relative expression level of APOE gene in C4-2 cell lines transfected by siRNA, and the knockdown efficiency was verified by qRT-PCR (B) and western blot (C). D: The protein expression levels of APOE detected by western blot in five pairs of paired RCC tissues and adjacent tissues. E: CCK-8 proliferation assay of wild type and APOE knockdown C4-2 cell lines. F: EdU proliferation assay of wild type and APOE knockdown C4-2 cell lines. The images were taken at 10x. G: Transwell assay of wild type and APOE knockdown C4-2 cell lines. The images were taken at 10x. G: Toxicity test of enzalutamide and apalutamide on wild type and APOE knockdown C4-2 cell lines, which compared by paired T-test. OD: optical density. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001