A-D. Relative acetylation of hepatic core histones in chicken after oral application of butyrate bolus. Butyrate was applied at a lower (0.25 g/kg BW) and a higher (1.25 g/kg BW) dose, relative acetylation ratios were compared to those of controls (considered as 100%). Acetylation ratios were determined considering relative protein expression levels of each histone and its acetylated form. Data are presented as mean ± SEM (n=6/group). *Significant difference, P<0.05; **Significant difference, P<0.01.