Representative bands as obtained by western blotting from isolated hepatocyte histones of chickens. Columns show the bands of control animals (C) and chicks after oral application of butyrate bolus at the dose of 0.25 g/kg body weight (BL) and the dose of 1.25 g/kg body weight (BH). The upper rows show the relative protein expression levels of total H2A, H2B, H3 and H4, respectively, while the bands specific for acetylated histones of the same animals can be seen below (AcH1-4). At histone H3, the upper band can be identified as the H3.1 isoform and the lower as H3.2. Western blots were done in duplicates for all histones.